Hypoxium

Isogenic cell lines

• Introduction
• Assays
• Isogenic Cell Lines

Current Disease Models

To investigate a particular target, compounds are often screened in large panels of cell lines with differential regulation of key oncogenes. Each cell line varies in multiple ways and commonly contains mutations or deregulation in multiple oncogenic pathways. Therefore understanding a drugs true mechanism of action can be complex.

Isogenic Disease Models

Paired isogenic cell lines provide a perfectly matched reference genetic background to determine the effects of that gene on the cells’ function and its responses to appropriately targeted agents. The only difference between the two components of the pair is the engineered genetic mutation of interest.

Hypoxium have licenced isogenic cell lines that are created by engineering a stable single or double knock-in/knock-out mutation of endogenous genes using proprietary gene engineering technology

Profiling Services

We are able to offer proliferation assays in isogenic paired cell lines as well as bespoke assay development. Isogenic cell lines currently available are listed below.

Cancer Gene	Parent cell	Notes
PI3Kα (H1047R)	Normal Breast Epithelium	Activating Kinase domain mutation
PI3Kα (E545K)	SW48 Colon Cancer Cells	Activation hinge domain mutation (Parental cells 'wild type' for PI3Kα)
EGFR (del E746-A750)	Normal Breast Epithelium	Activating mutation highly responsive to Gefitinib and Erlotinib
K-Ras (G13D)	Normal Breast Epithelium & SW48 Colon Cancer Cells	Activating mutation destroying GTPase activity leading to constitutive Ras-signalling
B-Raf (V600E)	Normal Breast Epithelium & SW48 Colon Cancer Cells	Activating Kinase domain mutation
PI3Ka (H1047R) & EGFR (del E746-A750)	Normal Breast Epithelium	Double knock-in of two cancer genes

For further details of this service, please contact us.

Isogenic cell lines have been licensed from Horizon Discovery. For further details on the technology, please visit their website.